Review



iq sybr green supermix  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    Bio-Rad iq sybr green supermix
    Iq Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 18218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iq+sybr+green+supermix/iQ+SYBR+Green+Supermix/pmc13049409-266-12-16
    Average 98 stars, based on 18218 article reviews
    iq sybr green supermix - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    SYBR Green Assay:

    Article Title: Immune-mediated microbial interference governs Borrelia colonization of the tick gut
    Article Snippet: .. PCRs were carried out using iQ SYBR Green Supermix (Bio-Rad Laboratories, Germany) following the manufacturer’s instructions. ..

    Article Title: Shape-defined poly(lactic acid)-nanohydroxyapatite composite microparticles modulate osteogenic differentiation in 3D microtissues of human mesenchymal stromal cells
    Article Snippet: Microtissues were collected on days 5, 10, and 20 for RNA isolation using Trizol reagent combined with PureLink RNA Mini Kit (Thermo Fisher Scientific). .. Then, the isolated RNA was used to amplify 20 ng of cDNA using iQ SYBR Green Supermix (Bio-Rad) in a CFX96 Real-Time PCR Detection Kit (Bio-Rad). ..

    Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxia in human brain organoids
    Article Snippet: .. Quantitative PCR analysis was performed using 2 μL of the diluted cDNA for 0.375 μL of primer set (10 μM) with iQ SYBR Green Supermix (Bio-Rad) in a total reaction volume of 12 μL. .. Amplification was conducted on a CFX96 RT-PCR detection system (Bio-Rad) with thermocycling protocol consisted of an initial denaturation at 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for 60 s, and 72°C for 30 s. Primer specificity was confirmed by performing melting curve analysis.

    Article Title: Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutations
    Article Snippet: 1 μg of RNA was reverse transcribed using BioRad iScript reverse transcriptase (Bio-Rad, 1708841) in a Bio-Rad C1000 Touch Thermal Cycler. .. 2 μL of the cDNA served as the template for qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and performed with a BioRad CFX96 Real-Time System. qPCR primers are shown in . ..

    Article Title: Micro-cold-forming: a simple, rapid, and inexpensive method for the fabrication of microcavities for 3D cell culture
    Article Snippet: HEPES, N -acetylcysteine, and ascorbic acid were obtained from Sigma-Aldrich. .. RNeasy Micro Kit was obtained from Qiagen and iScript cDNA Synthesis Kit and iQ SYBR Green Supermix were obtained from Bio-Rad. .. For forming the microcavities, a mold with 19 hexagonally arranged cylindrical holes, each with a diameter of 2 mm, a depth of 2 mm, and a minimum spacing of 0.25 mm between them, was designed using Autodesk Inventor 2018 (Autodesk) and precision-milled into a 4 mm thick SAE 316 stainless-steel plate (Xometry) (Fig. S1).

    Article Title: Immune infiltration is associated with distinct transcriptional states and metabolic profiles in chordomas
    Article Snippet: Transcriptor First Strand cDNA Synthesis Kit , Roche , https://lifescience.roche.com/global/en/products/others/transcriptor-first-strand-cdna-synthesis-kit-3683912.html. .. iQ SYBR Green Supermix , Bio-Rad , https://www.bio-rad.com/en-nl/product/iq-sybr-green-supermix?ID=M8G3SJ15. ..

    Article Title: Immune-mediated microbial interference governs Borrelia colonization of the tick gut
    Article Snippet: High-Capacity cDNA Reverse Transcription Kit , Applied Biosystems , Cat# 4368814. .. iQ SYBR Green Supermix , Bio-Rad , Cat# 1708882. .. QIAamp DNA Mini Kit , Qiagen , Cat# 51304.

    Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression
    Article Snippet: .. Reverse transcription was performed using RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019), M-MLV reverse transcriptase, 0.1 M DTT, 5× first-strand buffer (Invitrogen, 28025013), 10 mM deoxyribonucleotide triphosphate (dNTP) mix (Invitrogen, 362275), and random primers (Invitrogen, 48190011) at 37°C for 1 h, followed by 95°C for 1 min. Human SMN1 mRNA expression levels were quantified by qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and normalized to mouse β-actin using the 2 − ΔΔCt method ( ). .. Previously fixed and paraffin-embedded liver sections were incubated overnight at 4°C with a mouse monoclonal anti-SMN/Gemin 1 antibody (Abcam, ab5831) to detect SMN1 expression.

    Isolation:

    Article Title: Shape-defined poly(lactic acid)-nanohydroxyapatite composite microparticles modulate osteogenic differentiation in 3D microtissues of human mesenchymal stromal cells
    Article Snippet: Microtissues were collected on days 5, 10, and 20 for RNA isolation using Trizol reagent combined with PureLink RNA Mini Kit (Thermo Fisher Scientific). .. Then, the isolated RNA was used to amplify 20 ng of cDNA using iQ SYBR Green Supermix (Bio-Rad) in a CFX96 Real-Time PCR Detection Kit (Bio-Rad). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Shape-defined poly(lactic acid)-nanohydroxyapatite composite microparticles modulate osteogenic differentiation in 3D microtissues of human mesenchymal stromal cells
    Article Snippet: Microtissues were collected on days 5, 10, and 20 for RNA isolation using Trizol reagent combined with PureLink RNA Mini Kit (Thermo Fisher Scientific). .. Then, the isolated RNA was used to amplify 20 ng of cDNA using iQ SYBR Green Supermix (Bio-Rad) in a CFX96 Real-Time PCR Detection Kit (Bio-Rad). ..

    Article Title: Female metabolic resilience and male-biased protein quality defects under hypoxia in human brain organoids
    Article Snippet: .. Quantitative PCR analysis was performed using 2 μL of the diluted cDNA for 0.375 μL of primer set (10 μM) with iQ SYBR Green Supermix (Bio-Rad) in a total reaction volume of 12 μL. .. Amplification was conducted on a CFX96 RT-PCR detection system (Bio-Rad) with thermocycling protocol consisted of an initial denaturation at 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 60°C for 60 s, and 72°C for 30 s. Primer specificity was confirmed by performing melting curve analysis.

    Article Title: Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutations
    Article Snippet: 1 μg of RNA was reverse transcribed using BioRad iScript reverse transcriptase (Bio-Rad, 1708841) in a Bio-Rad C1000 Touch Thermal Cycler. .. 2 μL of the cDNA served as the template for qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and performed with a BioRad CFX96 Real-Time System. qPCR primers are shown in . ..

    Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression
    Article Snippet: .. Reverse transcription was performed using RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019), M-MLV reverse transcriptase, 0.1 M DTT, 5× first-strand buffer (Invitrogen, 28025013), 10 mM deoxyribonucleotide triphosphate (dNTP) mix (Invitrogen, 362275), and random primers (Invitrogen, 48190011) at 37°C for 1 h, followed by 95°C for 1 min. Human SMN1 mRNA expression levels were quantified by qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and normalized to mouse β-actin using the 2 − ΔΔCt method ( ). .. Previously fixed and paraffin-embedded liver sections were incubated overnight at 4°C with a mouse monoclonal anti-SMN/Gemin 1 antibody (Abcam, ab5831) to detect SMN1 expression.

    cDNA Synthesis:

    Article Title: Micro-cold-forming: a simple, rapid, and inexpensive method for the fabrication of microcavities for 3D cell culture
    Article Snippet: HEPES, N -acetylcysteine, and ascorbic acid were obtained from Sigma-Aldrich. .. RNeasy Micro Kit was obtained from Qiagen and iScript cDNA Synthesis Kit and iQ SYBR Green Supermix were obtained from Bio-Rad. .. For forming the microcavities, a mold with 19 hexagonally arranged cylindrical holes, each with a diameter of 2 mm, a depth of 2 mm, and a minimum spacing of 0.25 mm between them, was designed using Autodesk Inventor 2018 (Autodesk) and precision-milled into a 4 mm thick SAE 316 stainless-steel plate (Xometry) (Fig. S1).

    Reverse Transcription:

    Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression
    Article Snippet: .. Reverse transcription was performed using RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019), M-MLV reverse transcriptase, 0.1 M DTT, 5× first-strand buffer (Invitrogen, 28025013), 10 mM deoxyribonucleotide triphosphate (dNTP) mix (Invitrogen, 362275), and random primers (Invitrogen, 48190011) at 37°C for 1 h, followed by 95°C for 1 min. Human SMN1 mRNA expression levels were quantified by qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and normalized to mouse β-actin using the 2 − ΔΔCt method ( ). .. Previously fixed and paraffin-embedded liver sections were incubated overnight at 4°C with a mouse monoclonal anti-SMN/Gemin 1 antibody (Abcam, ab5831) to detect SMN1 expression.

    Recombinant:

    Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression
    Article Snippet: .. Reverse transcription was performed using RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019), M-MLV reverse transcriptase, 0.1 M DTT, 5× first-strand buffer (Invitrogen, 28025013), 10 mM deoxyribonucleotide triphosphate (dNTP) mix (Invitrogen, 362275), and random primers (Invitrogen, 48190011) at 37°C for 1 h, followed by 95°C for 1 min. Human SMN1 mRNA expression levels were quantified by qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and normalized to mouse β-actin using the 2 − ΔΔCt method ( ). .. Previously fixed and paraffin-embedded liver sections were incubated overnight at 4°C with a mouse monoclonal anti-SMN/Gemin 1 antibody (Abcam, ab5831) to detect SMN1 expression.

    Expressing:

    Article Title: Orthogonal characterization of rAAV reveals vector attributes that drive ITR repair, self-complementary genome formation, and transgene expression
    Article Snippet: .. Reverse transcription was performed using RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019), M-MLV reverse transcriptase, 0.1 M DTT, 5× first-strand buffer (Invitrogen, 28025013), 10 mM deoxyribonucleotide triphosphate (dNTP) mix (Invitrogen, 362275), and random primers (Invitrogen, 48190011) at 37°C for 1 h, followed by 95°C for 1 min. Human SMN1 mRNA expression levels were quantified by qPCR using iQ SYBR Green Supermix (Bio-Rad, 1708882) and normalized to mouse β-actin using the 2 − ΔΔCt method ( ). .. Previously fixed and paraffin-embedded liver sections were incubated overnight at 4°C with a mouse monoclonal anti-SMN/Gemin 1 antibody (Abcam, ab5831) to detect SMN1 expression.



    Similar Products

    98
    Bio-Rad iq sybr green supermix
    Iq Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iq+sybr+green+supermix/iQ+SYBR+Green+Supermix/pmc13049409-266-12-16
    Average 98 stars, based on 1 article reviews
    iq sybr green supermix - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    Bio-Rad qpcr assay
    Qpcr Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iq+sybr+green+supermix/iQ+SYBR+Green+Supermix/pmc13049938-274-28-35
    Average 98 stars, based on 1 article reviews
    qpcr assay - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    98
    Bio-Rad sybr green supermix
    Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iq+sybr+green+supermix/iQ+SYBR+Green+Supermix/pmc13145380-116-17-20
    Average 98 stars, based on 1 article reviews
    sybr green supermix - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    99
    Bio-Rad iqtm sybr green supermix
    Iqtm Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iq+sybr+green+supermix/IQ+Sybr+Green+Super+Mix/pmc12994450-219-18-24
    Average 99 stars, based on 1 article reviews
    iqtm sybr green supermix - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results